Protein Assays remain quite popular in life science research to determine the level of thyroid hormones in the blood

 

Protein assays are available in three categories namely semi-experimental, fully-experimental, and fluorescence-based. There is no truly perfect protein assays method, per se, as every method is capable of providing results that are variable and depend on the type of protein being tested, the type of material being used, and the expertise of the personnel testing the samples. However, there is a highly efficient protein assays market segmented based on absorbance-based and fluorescence-based protein assays.

Absorbance-based protein assays are the most commonly utilized in scientific research. They are based on a method of detecting individual molecules of different proteins using highly sensitive and specialized optical fibers. Fluorescence-based protein assays work by allowing proteins to absorb Fluorinated proteins through the emission of green light. These types of proteins can be detected using a fluoroscope or other fluorescent-based instrument. The absorbance-based protein assays are highly sensitive to changes in the concentration and temperature of the protein content. For this reason, protein assays are commonly used in the studies of the absorption of metals like lead, mercury, cadmium, and aluminum. These particular types of assays are sensitive to the effects of temperature, metal ions, and even small changes in the amino acid sequence of the analyte.

There are three basic classifications of absorbance-based protein assays. The first two, known as first-order or first-step methods, include a single sample collection at a time from the same area. The third and most advanced type of protein assay uses two or more samples at a time in a process called tandem dilution. The tandem-dilution method allows for the separation of approximately equal amounts of the analyte from samples taken at different times. The absorbance-based protein assays with the tandem-dilution system are expected to make up the largest portion of the market. Recently, in October 2020, Synthace launched a new software capability for streamlining enzyme-linked immunosorbent assay (ELISA).